Ovine oocytes display a similar germinal vesicle configuration and global DNA methylation at prepubertal and adult ages

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dc.bibliographicCitation.titleTheriogenology: An International Journal Of Animal Reproductionen
dc.contributor.authorCocero, M.es_ES
dc.contributor.authorMarigorta, P.es_ES
dc.contributor.authorNovillo, F.es_ES
dc.contributor.authorFolch Pera, Josées_ES
dc.contributor.authorSánchez Ruiz, Pilares_ES
dc.contributor.authorAlabart Alvarez, José Luises_ES
dc.contributor.authorLahoz Crespo, Belénes_ES
dc.coverage.spatialCiencia Animales_ES
dc.date.accessioned2025-02-19T09:46:46Z
dc.date.available2025-02-19T09:46:46Z
dc.date.issued2019es_ES
dc.date.updated2025-02-07T11:12:30Z
dc.description.abstract© 2019 Elsevier Inc. Epigenetic mechanisms are thought to be involved in the reduced developmental capacity of early prepubertal ewe oocytes compared to their adult counterparts. In this study, we have analyzed the global DNA methylation pattern and in vitro meiotic and developmental competence of oocytes at the germinal vesicle (GV) stage obtained from adult and 3-month-old donors. All oocytes were aspirated from antral follicles with a diameter ≥3 mm, and DNA methylation on 5-methylcytosine was detected by immunofluorescence using an anti-methyl cytosine antibody. The main global chromatin configuration pattern shown by both prepubertal and adult ovine oocytes corresponded to condensed chromatin localized close to the nuclear envelope (the SNE pattern). Immunofluorescence showed that a global bright nuclear staining of 5-methylcytosine (5-mC) occurred in all germinal vesicle stage oocytes and matched the propidium iodide staining pattern. The total fluorescence intensity values of lamb GVs were not lower than those observed in adult GVs. The meiotic competence and cleavage rates were similar in adult and prepubertal oocytes, however, the developmental competence of embryos to reach blastocysts was higher for adult oocytes than lamb oocytes (p<0.0001). In conclusion, our results indicate that adult-size oocytes derived from 3 to 4 month old prepubertal ewes show similar GV morphology and DNA methylation staining patterns to those obtained from adult animals, despite exhibiting a lower developmental competence.en
dc.description.otherAntral follicleen
dc.description.otherSheepen
dc.description.otherChromatinen
dc.description.otherEpigeneticen
dc.description.otherEmbryoen
dc.description.otherBlastocysten
dc.description.sponsorshipThis work was funded was suportad by INIA, Spanish Ministry of Economy and Competitiveness (RTA2011-00128-C02 and RTA2013-00041-C02)es_ES
dc.description.statusPublishedes_ES
dc.identifier.citationCocero, Maria J; Marigorta, P.; Novillo, F.; Folch, J.; Sanchez, P.; Alabart, J. L; Lahoz, B. Ovine oocytes display a similar germinal vesicle configuration and global DNA methylation at prepubertal and adult ages. Theriogenology, 2019, 138, 154-163
dc.identifier.issn0093691X
dc.identifier.urihttp://hdl.handle.net/10532/7518
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1016/j.theriogenology.2019.07.011es_ES
dc.relation.urihttps://doi.org/10.1016/j.theriogenology.2019.07.011es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocOvocitoses
dc.subject.agrovocOvejaes
dc.subject.agrovocEpigenéticoes
dc.subject.agrovocBlastocistoses
dc.subject.agrovocCromatinaes
dc.subject.otherAntral follicle
dc.subject.otherBlastocyst
dc.subject.otherChromatin
dc.subject.otherCHROMATIN CONFIGURATION
dc.subject.otherDevelopmental competence
dc.subject.otherEmbryo
dc.subject.otherEpigenetic
dc.subject.otherJuvenile
dc.subject.othermaturation
dc.subject.otherMorphology
dc.subject.otherOOGENESIS
dc.subject.otherOrganization
dc.subject.otherProgression
dc.subject.othersheep
dc.subject.otherSHEEP OOCYTES
dc.subject.otherAge Factors
dc.subject.otherAging
dc.subject.otherAnimals
dc.subject.otherantral follicle
dc.subject.otherBLASTOCYST
dc.subject.otherCell Nucleus
dc.subject.otherChromatin
dc.subject.otherDna Methylation
dc.subject.otherEmbryo
dc.subject.otherEpigenesis, Genetic
dc.subject.otherEpigenetic
dc.subject.otherFemale
dc.subject.otherIn Vitro Oocyte Maturation Techniques
dc.subject.otherIn-Vitro
dc.subject.otherMeiosis
dc.subject.otherOocyte Donation
dc.subject.otherOocytes
dc.subject.othersexual maturation
dc.subject.otherSheep
dc.titleOvine oocytes display a similar germinal vesicle configuration and global DNA methylation at prepubertal and adult agesen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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