Cam β-NGF Supplementation: A Promising Approach to Preserve Dromedary Camel Sperm Quality During Short-term Storage
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Date
2025-10-31
Authors
Doghbri, LamiaAtigui, Moufida
Salhi, Imed
Carvajal Serna, Melissa
Casao, Adriana
Dbara, Mohamed
Pérez Pe. Rosaura
Hammadi, Mohamed
Kdidi, Samia
Journal Title
Journal ISSN
Volume Title
Publisher
Bentham Open
Typology
texto , revista , artículo , artículo originalAbstract
Introduction
The role of camel βeta-Nerve Growth Factor protein (Cam β-NGF), isolated from camel seminal plasma, in preserving sperm quality remains poorly defined. This study aimed to evaluate the impact of Cam β-NGF on mass motility, viability, and membrane integrity in dromedary (Camelus dromedarius) semen stored for 24 hours at 4 °C.
Methods
The semen samples were treated with varying concentrations of Cam β-NGF (10, 100, 500, and 1000 ng/mL) after preservation. Ten semen samples with mass motility ≥ 3 and viability ≥ 60% were selected, washed, suspended in HBSS, and treated with TCF-EY. After 24 hours of storage at 4 °C, samples were maintained at 36 °C with Cam β-NGF supplementation for 60 minutes. Mass motility, viability, and membrane integrity were assessed at T0, T30, and T60 minutes.
Results
The results revealed a significant decline in sperm quality traits after 24 hours of storage at 4 °C. However, mass motility was conserved after 30 minutes of incubation with 1000 ng/mL and 500 ng/mL of Cam β-NGF (p < 0.05). On the other hand, viability was significantly higher after 60 minutes of incubation with 1000, 500, and 100 ng/mL of Cam β-NGF compared to the control extender and 10 ng/mL supplementation (p < 0.05). Additionally, membrane integrity was significantly maintained at T30 and T60 minutes with 1000, 500, and 100 ng/mL of Cam β-NGF (p < 0.05).
Discussion
Short‐term storage at 4 °C markedly reduced dromedary camel sperm motility, viability, and membrane integrity, consistent with cold‐induced oxidative stress. Adding purified Cam β-NGF dose-dependently preserved these parameters over 1 h of incubation, indicating its value as a protective supplement for semen storage.
Conclusion
Cam β-NGF helps protect and maintain sperm traits during short-term storage; it seems to reduce alterations that might lower sperm quality. Thus, supplementing semen with Cam β-NGF may help maintain sperm quality, offering potential benefits for artificial insemination programs in dromedaries.
Description
Keywords
Bibliographic citation
Doghbri, L., Atigui, M., Salhi, I., Carvajal-Serna, M., Casao, A., Dbara, M., Péréz-Pé, R., Hammadi, M., & Kdidi, S. (2025). Cam β-NGF Supplementation: A Promising Approach to Preserve Dromedary Camel Sperm Quality During Short-term Storage. https://doi.org/10.2174/0118740707389100251014053002
AGROVOC subjects
Camelus dromedariusSemen
Espermatozoo
Ai (artificial insemination)
Factor de crecimiento
Sponsorship
The Tunisian Ministry of Higher Education and Scientific Research (Contract program 2020–2023 of LR16IRA04), Tunisia funded this work. The hormonal analysis, the immunofluorescence research, and the Western blotting were funded by CICYT AGL-2017-83799-R and DGA A07 17R.