Cam β-NGF Supplementation: A Promising Approach to Preserve Dromedary Camel Sperm Quality During Short-term Storage

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dc.contributor.authorDoghbri, Lamia
dc.contributor.authorAtigui, Moufida
dc.contributor.authorSalhi, Imed
dc.contributor.authorCarvajal Serna, Melissa
dc.contributor.authorCasao, Adriana
dc.contributor.authorDbara, Mohamed
dc.contributor.authorPérez Pe. Rosaura
dc.contributor.authorHammadi, Mohamed
dc.contributor.authorKdidi, Samia
dc.contributor.orcidCarvajal Serna, Melissa [0000-0003-3929-5064]
dc.date.accessioned2025-12-23T07:55:06Z
dc.date.available2025-12-23T07:55:06Z
dc.date.issued2025-10-31
dc.date.updated2025-11-20T10:58:58Z
dc.description.abstractIntroduction The role of camel βeta-Nerve Growth Factor protein (Cam β-NGF), isolated from camel seminal plasma, in preserving sperm quality remains poorly defined. This study aimed to evaluate the impact of Cam β-NGF on mass motility, viability, and membrane integrity in dromedary (Camelus dromedarius) semen stored for 24 hours at 4 °C. Methods The semen samples were treated with varying concentrations of Cam β-NGF (10, 100, 500, and 1000 ng/mL) after preservation. Ten semen samples with mass motility ≥ 3 and viability ≥ 60% were selected, washed, suspended in HBSS, and treated with TCF-EY. After 24 hours of storage at 4 °C, samples were maintained at 36 °C with Cam β-NGF supplementation for 60 minutes. Mass motility, viability, and membrane integrity were assessed at T0, T30, and T60 minutes. Results The results revealed a significant decline in sperm quality traits after 24 hours of storage at 4 °C. However, mass motility was conserved after 30 minutes of incubation with 1000 ng/mL and 500 ng/mL of Cam β-NGF (p < 0.05). On the other hand, viability was significantly higher after 60 minutes of incubation with 1000, 500, and 100 ng/mL of Cam β-NGF compared to the control extender and 10 ng/mL supplementation (p < 0.05). Additionally, membrane integrity was significantly maintained at T30 and T60 minutes with 1000, 500, and 100 ng/mL of Cam β-NGF (p < 0.05). Discussion Short‐term storage at 4 °C markedly reduced dromedary camel sperm motility, viability, and membrane integrity, consistent with cold‐induced oxidative stress. Adding purified Cam β-NGF dose-dependently preserved these parameters over 1 h of incubation, indicating its value as a protective supplement for semen storage. Conclusion Cam β-NGF helps protect and maintain sperm traits during short-term storage; it seems to reduce alterations that might lower sperm quality. Thus, supplementing semen with Cam β-NGF may help maintain sperm quality, offering potential benefits for artificial insemination programs in dromedaries.
dc.description.peerreviewedSi
dc.description.sponsorshipThe Tunisian Ministry of Higher Education and Scientific Research (Contract program 2020–2023 of LR16IRA04), Tunisia funded this work. The hormonal analysis, the immunofluorescence research, and the Western blotting were funded by CICYT AGL-2017-83799-R and DGA A07 17R.
dc.identifier.citationDoghbri, L., Atigui, M., Salhi, I., Carvajal-Serna, M., Casao, A., Dbara, M., Péréz-Pé, R., Hammadi, M., & Kdidi, S. (2025). Cam β-NGF Supplementation: A Promising Approach to Preserve Dromedary Camel Sperm Quality During Short-term Storage. https://doi.org/10.2174/0118740707389100251014053002
dc.identifier.doi10.2174/0118740707389100251014053002
dc.identifier.issn1874-0707
dc.identifier.urihttp://doi.org/10.2174/0118740707389100251014053002
dc.identifier.urihttps://hdl.handle.net/10532/8073
dc.language.isoen
dc.publisherBentham Open
dc.relation.citaSi
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spainen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.agrovocCamelus dromedarius
dc.subject.agrovocSemen
dc.subject.agrovocEspermatozoo
dc.subject.agrovocAi (artificial insemination)
dc.subject.agrovocFactor de crecimiento
dc.subject.sdgVida de ecosistemas terrestres
dc.titleCam β-NGF Supplementation: A Promising Approach to Preserve Dromedary Camel Sperm Quality During Short-term Storage
dc.typetexto
dc.typerevista
dc.typeartículo
dc.typeartículo original
dc.type.hasVersionversión publicada

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