The extradomain A of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice
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Date
2012
Authors
San Román, BeatrizGarrido, Victoria
Muñoz Álvaro, Pilar María
Arribillaga, Laura
García, Begoña
Andrés, Ximena de
Zabaleta, Virginia
Mansilla, Cristina
Farrán, Inmaculada
Lasa, Iñigo
Andrés, Damián de
Amorena Zabalza, Beatriz
Lasarte, Juan José
Grilló Dolset, María Jesús
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ArticleAbstract
The Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein
with viral and tumor antigens, but its effect when administered with bacteria has not been
assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against
Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidis). Since
lipopolysaccharide (LPS) is a major virulence factor and the LPS O-polysaccharide (O-PS) is
the immunodominant antigen in serological diagnostic tests, Salmonella mutants lacking OPS
(rough mutants) represent an interesting approach for developing new vaccines and
diagnostic tests to differentiate infected and vaccinated animals (DIVA tests). Here, antigenic
preparations (hot-saline extracts and formalin-inactivated bacterins) from two Salmonella
Enteritidis rough mutants, carrying either intact (SEΔwaaL) or deep-defective (SEΔgal) LPSCore,
were used in combination with EDA. Biotinylated bacterins, in particular SEΔwaaL
bacterin, decorated with EDAvidin (EDA and streptavidin fusion protein) improved the
protection conferred by hot-saline or bacterins alone and prevented significantly the virulent
infection at least to the levels of live attenuated rough mutants. These findings demonstrate
the adjuvant effect of EDAvidin when administered with biotinylated bacterins from
Salmonella Enteritidis lacking O-polysaccharide and the usefulness of BEDA-SEΔwaaL as
non-live vaccine in the mouse model.
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Bibliographic citation
Beatriz San Roman, Victoria Garrido, Pilar-Maria Munoz, Laura Arribillaga, Begona Garcia, Ximena De Andres, Virginia Zabaleta, Cristina Mansilla, Inmaculada Farran, Inigo Lasa, Damian De Andres, Beatriz Amorena, Juan-Jose Lasarte and Maria-Jesus Grillo. “The extradomain A of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice”. Veterinary Research 2012, 43:31
Other field subjects
VacunaSalmonella
Experimentación en laboratorio
Producción y sanidad animal