The extradomain A of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice

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dc.contributor.authorSan Román, Beatriz
dc.contributor.authorGarrido, Victoria
dc.contributor.authorMuñoz Álvaro, Pilar María
dc.contributor.authorArribillaga, Laura
dc.contributor.authorGarcía, Begoña
dc.contributor.authorAndrés, Ximena de
dc.contributor.authorZabaleta, Virginia
dc.contributor.authorMansilla, Cristina
dc.contributor.authorFarrán, Inmaculada
dc.contributor.authorLasa, Iñigo
dc.contributor.authorAndrés, Damián de
dc.contributor.authorAmorena Zabalza, Beatriz
dc.contributor.authorLasarte, Juan José
dc.contributor.authorGrilló Dolset, María Jesús
dc.date.accessioned2012-05-10T07:54:20Z
dc.date.available2012-05-10T07:54:20Z
dc.date.issued2012
dc.description.abstractThe Extradomain A from fibronectin (EDA) has an immunomodulatory role as fusion protein with viral and tumor antigens, but its effect when administered with bacteria has not been assessed. Here, we investigated the adjuvant effect of EDA in mice immunizations against Salmonella enterica subspecies enterica serovar Enteritidis (Salmonella Enteritidis). Since lipopolysaccharide (LPS) is a major virulence factor and the LPS O-polysaccharide (O-PS) is the immunodominant antigen in serological diagnostic tests, Salmonella mutants lacking OPS (rough mutants) represent an interesting approach for developing new vaccines and diagnostic tests to differentiate infected and vaccinated animals (DIVA tests). Here, antigenic preparations (hot-saline extracts and formalin-inactivated bacterins) from two Salmonella Enteritidis rough mutants, carrying either intact (SEΔwaaL) or deep-defective (SEΔgal) LPSCore, were used in combination with EDA. Biotinylated bacterins, in particular SEΔwaaL bacterin, decorated with EDAvidin (EDA and streptavidin fusion protein) improved the protection conferred by hot-saline or bacterins alone and prevented significantly the virulent infection at least to the levels of live attenuated rough mutants. These findings demonstrate the adjuvant effect of EDAvidin when administered with biotinylated bacterins from Salmonella Enteritidis lacking O-polysaccharide and the usefulness of BEDA-SEΔwaaL as non-live vaccine in the mouse model.es_ES
dc.identifier.citationBeatriz San Roman, Victoria Garrido, Pilar-Maria Munoz, Laura Arribillaga, Begona Garcia, Ximena De Andres, Virginia Zabaleta, Cristina Mansilla, Inmaculada Farran, Inigo Lasa, Damian De Andres, Beatriz Amorena, Juan-Jose Lasarte and Maria-Jesus Grillo. “The extradomain A of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in mice”. Veterinary Research 2012, 43:31 
dc.identifier.urihttp://hdl.handle.net/10532/1862
dc.language.isoenes_ES
dc.relation.publisherversionhttp://www.veterinaryresearch.org/content/pdf/1297-9716-43-31.pdf
dc.subject.otherVacuna
dc.subject.otherSalmonella
dc.subject.otherExperimentación en laboratorio
dc.subject.otherProducción y sanidad animales_ES
dc.titleThe extradomain A of fibronectin enhances the efficacy of lipopolysaccharide defective Salmonella bacterins as vaccines in micees_ES
dc.typeArticlees_ES

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