A Review on the Methodology and Use of the Pregnant Mouse Model in the Study of Brucella Reproductive Pathogenesis and Its Abortifacient Effect
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Date
2024
Authors
Elizalde Bielsa, AitorMuñoz Álvaro, Pilar María
Zúñiga Ripa, Amaia
Conde Álvarez, Raquel
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articleArticle
Abstract
Brucellosis is one of the most common and widespread bacterial zoonoses and is caused by Gram-negative bacteria belonging to the genus Brucella. These organisms are able to infect and replicate within the placenta, resulting in abortion, one of the main clinical signs of brucellosis. Although the mouse model is widely used to study Brucella virulence and, more recently, to evaluate the protection of new vaccines, there is no clear consensus on the experimental conditions (e.g., mouse strains, doses, routes of inoculation, infection/pregnancy time) and the natural host reproducibility of the pregnant mouse model for reproductive brucellosis. This lack of consensus calls for a review that integrates the major findings regarding the effect of Brucella wild-type and vaccine strains infections on mouse pregnancy. We found sufficient evidence on the utility of the pregnant mouse model to study Brucella-induced placentitis and abortion and propose suitable experimental conditions (dose, time of infection) and pregnancy outcome readouts for B. abortus and B. melitensis studies. Finally, we discuss the utility and limitations of the pregnant mouse as a predictive model for the abortifacient effect of live Brucella vaccines.
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Elizalde-Bielsa, A., Muñoz, P. M., Zúñiga-Ripa, A., & Conde-Álvarez, R. (2024). A Review on the Methodology and Use of the Pregnant Mouse Model in the Study of Brucella Reproductive Pathogenesis and Its Abortifacient Effect. Microorganisms, 12(5), Article 5. https://doi.org/10.3390/microorganisms12050866
AGROVOC subjects
BrucellaRatón
Animales de laboratorio
Placenta
Embriones animales
Patogénesis
Aborto
Vacuna
Other field subjects
AbortoAnimales de laboratorio
Brucella
Embriones Animales
Patogénesis
Placenta
Ratón
Vacuna
Sponsorship
Esta revisión se ha realizado en el marco de los proyectos PID2019-107601RB-C31 y PID2019-107601RA-C32 financiados por MCIN/AEI/10.1303910.13039/501100011033. El trabajo de A.E-B fue apoyado por una Beca de Doctorado financiada por el Instituto de Salud Tropical Universidad de Navarra (ISTUN) financiadores (Fundación la CAIXA [LCF/PR/PR13/51080005], Fundación María Francisca de Roviralta, y Ubesol) y el proyecto REPRODIVAC bajo el Acuerdo de Subvención No. 10106081 a través de la Convocatoria HORIZON-CL6-2021-FARM2FORK-01-06. El trabajo de P.M.M (CITA) también fue apoyado por el Gobierno de Aragón (Grupo de Investigación A21_23R).