The efficiency of in vitro ovine embryo production using an undefined or a defined maturation medium is determined by the source of the oocyte
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Date
2024
Authors
Cocero Oviedo, Mª JesúsAlabart Alvarez, José Luis
Hammami, S.
Martí Jiménez, José Ignacio
Lahoz Crespo, Belén
Sánchez Ruiz, Pilar
Echegoyen Pérez, Elías
Beckers, J.F.
Folch Pera, José
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Abstract
In vitro oocyte maturation can be influenced by oocyte source and maturation media composition. The aim of the present study was to compare the efficiency of a defined in vitro maturation medium (TCM199 supplemented with cysteamine and epidermal growth factor; Cys + EGF) with an undefined medium (TCM199 supplemented with follicle-stimulating hormone and follicular fluid; FSH + FF) for in vitro production (IVP) of ovine embryos, using oocytes obtained by laparoscopic ovum pick-up from FSH-stimulated [n=11; 158 cumulus-oocyte complexes (COCs)] and non-stimulated (n=16; 120 COCs) live ewes, as well as abattoir-derived oocytes (170 COCs). The produced blastocysts were vitrified and some of them were transferred to synchronized recipients. The best and the worst final yields of embryo IVP observed in this study were obtained using oocytes from FSH-stimulated ewes matured in FSH + FF (41.3%; 33/80) and in Cys + EGF (19.2%; 15/78) medium, respectively (p<0.01). No significant differences between both media were attained in the blastocyst development rate or in the final yield of embryo IVP using oocytes from non-stimulated ewes or abattoir-derived oocytes. The overall in vivo survival rate of the transferred vitrified blastocysts was 13.1% (8/61), without significant differences between oocyte sources or maturation media. In conclusion, under the experimental conditions of the present study, TCM199 supplemented with cysteamine and EGF is a convenient defined maturation medium for IVP of embryos from oocytes of live non-stimulated ewes or from oocytes of abattoir-derived ovaries. However, the best final yield of embryo IVP observed in this study was attained when oocytes came from FSH-stimulated donors and TCM199 was supplemented with FSH and follicular fluid.© 2010 Blackwell Verlag GmbH.
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Bibliographic citation
Cocero, M. J.; Alabart, J. L.; Hammami, S.; Beckers, J. F. The efficiency of in vitro ovine embryo production using an undefined or a defined maturation medium is determined by the source of the oocyte. Reproduction In Domestic Animals, 2011, 46, 3, 463-70
AGROVOC subjects
OvinoProducción de embriones in vitro
Ovocito
Embrión animal
Fertilidad
Other field subjects
AnimalsBLASTOCYST
Bovine
Capacity
Cells, Cultured
Cryopreservation
Culture Media
DEVELOPMENTAL COMPETENCE
Embryo Culture Techniques
Embryo Transfer
embryonic development
Epidermal-Growth-Factor
Female
fertilization
Fertilization in Vitro
Fluid
Follicle Stimulating Hormone
Follicle-Stimulating-Hormone
Follicular Fluid
Of-The-Art
Oocytes
OVUM PICK-UP
Pregnancy
Sheep
SHEEP OOCYTES
Sponsorship
This work was supported by M de Educacio´n y Ciencia (Project
AGL2005-08277) and IAMZ-CIHEAM (grant for S. Hammami).