The efficiency of in vitro ovine embryo production using an undefined or a defined maturation medium is determined by the source of the oocyte

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dc.bibliographicCitation.endpage470es_ES
dc.bibliographicCitation.issue3es_ES
dc.bibliographicCitation.stpage463es_ES
dc.bibliographicCitation.titleReproduction In Domestic Animalsen
dc.bibliographicCitation.volume46es_ES
dc.contributor.authorCocero Oviedo, Mª Jesúses_ES
dc.contributor.authorAlabart Alvarez, José Luises_ES
dc.contributor.authorHammami, S.es_ES
dc.contributor.authorMartí Jiménez, José Ignacioes_ES
dc.contributor.authorLahoz Crespo, Belénes_ES
dc.contributor.authorSánchez Ruiz, Pilares_ES
dc.contributor.authorEchegoyen Pérez, Elíases_ES
dc.contributor.authorBeckers, J.F.es_ES
dc.contributor.authorFolch Pera, Josées_ES
dc.coverage.temporalCiencia animales_ES
dc.date.accessioned2025-01-31T12:05:57Z
dc.date.available2025-01-31T12:05:57Z
dc.date.issued2024es_ES
dc.date.updated2025-01-31T12:03:05Z
dc.description.abstractIn vitro oocyte maturation can be influenced by oocyte source and maturation media composition. The aim of the present study was to compare the efficiency of a defined in vitro maturation medium (TCM199 supplemented with cysteamine and epidermal growth factor; Cys + EGF) with an undefined medium (TCM199 supplemented with follicle-stimulating hormone and follicular fluid; FSH + FF) for in vitro production (IVP) of ovine embryos, using oocytes obtained by laparoscopic ovum pick-up from FSH-stimulated [n=11; 158 cumulus-oocyte complexes (COCs)] and non-stimulated (n=16; 120 COCs) live ewes, as well as abattoir-derived oocytes (170 COCs). The produced blastocysts were vitrified and some of them were transferred to synchronized recipients. The best and the worst final yields of embryo IVP observed in this study were obtained using oocytes from FSH-stimulated ewes matured in FSH + FF (41.3%; 33/80) and in Cys + EGF (19.2%; 15/78) medium, respectively (p<0.01). No significant differences between both media were attained in the blastocyst development rate or in the final yield of embryo IVP using oocytes from non-stimulated ewes or abattoir-derived oocytes. The overall in vivo survival rate of the transferred vitrified blastocysts was 13.1% (8/61), without significant differences between oocyte sources or maturation media. In conclusion, under the experimental conditions of the present study, TCM199 supplemented with cysteamine and EGF is a convenient defined maturation medium for IVP of embryos from oocytes of live non-stimulated ewes or from oocytes of abattoir-derived ovaries. However, the best final yield of embryo IVP observed in this study was attained when oocytes came from FSH-stimulated donors and TCM199 was supplemented with FSH and follicular fluid.© 2010 Blackwell Verlag GmbH.en
dc.description.sponsorshipThis work was supported by M de Educacio´n y Ciencia (Project AGL2005-08277) and IAMZ-CIHEAM (grant for S. Hammami).
dc.description.statusPublishedes_ES
dc.identifier.citationCocero, M. J.; Alabart, J. L.; Hammami, S.; Beckers, J. F. The efficiency of in vitro ovine embryo production using an undefined or a defined maturation medium is determined by the source of the oocyte. Reproduction In Domestic Animals, 2011, 46, 3, 463-70
dc.identifier.issn09366768
dc.identifier.urihttp://hdl.handle.net/10532/7465
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1111/j.1439-0531.2010.01690.xes_ES
dc.relation.urihttps://doi.org/10.1111/j.1439-0531.2010.01690.xes_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Spaines_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocOvinoes_ES
dc.subject.agrovocProducción de embriones in vitroes_ES
dc.subject.agrovocOvocitoes_ES
dc.subject.agrovocEmbrión animales_ES
dc.subject.agrovocFertilidades_ES
dc.subject.otherAnimals
dc.subject.otherBLASTOCYST
dc.subject.otherBovine
dc.subject.otherCapacity
dc.subject.otherCells, Cultured
dc.subject.otherCryopreservation
dc.subject.otherCulture Media
dc.subject.otherDEVELOPMENTAL COMPETENCE
dc.subject.otherEmbryo Culture Techniques
dc.subject.otherEmbryo Transfer
dc.subject.otherembryonic development
dc.subject.otherEpidermal-Growth-Factor
dc.subject.otherFemale
dc.subject.otherfertilization
dc.subject.otherFertilization in Vitro
dc.subject.otherFluid
dc.subject.otherFollicle Stimulating Hormone
dc.subject.otherFollicle-Stimulating-Hormone
dc.subject.otherFollicular Fluid
dc.subject.otherOf-The-Art
dc.subject.otherOocytes
dc.subject.otherOVUM PICK-UP
dc.subject.otherPregnancy
dc.subject.otherSheep
dc.subject.otherSHEEP OOCYTES
dc.titleThe efficiency of in vitro ovine embryo production using an undefined or a defined maturation medium is determined by the source of the oocyteen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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