Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition
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Date
2024
Authors
Millán-Laleona, AdriánCebollada, Pilar
Caprioli, Giovanni
Piatti, Diletta
Maggi, Filippo
Pina Sobrino, Ana
Gomez-Rincon, Carlota
López, Victor
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Abstract
Sustainability in the fruit industry and protection of local cultivars are nowadays important tasks for consumers and authorities. Phenolic compounds are interesting to explore because they play an important role in fruit-related health benefits. The phenolic profile of the regional and commercial Spanish apple cultivars was performed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). For a deeper understanding of their anti-inflammatory and antioxidant potential, the 5-lipoxigenase inhibition and NO? scavenging were evaluated. Moreover, cellular viability was determined to perform MTT assay in HepG2 and HeLa cell lines to discard cytotoxicity. The results showed that procyanidin B2 (PB2), 3-caffeoylquinic acid, epicatechin and 4-hydroxy benzoic acid were the most abundant phenolic compounds. Autochthonous apple cultivars showed better 5-LOX/NO? inhibition and nontoxic effects in the tested cell lines with an interesting profile of phenolic compounds. Local autochthonous apples can be considered a better source of bioactive compounds than the commercial samples.
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Bibliographic citation
Millán-Laleona, A., Cebollada, P., Caprioli, G., Piatti, D., Maggi, F., Pina, A., Gómez-Rincón, C., & López, V. Valorization of local regional apple (Malus domestica Borkh.) cultivars versus commercial samples from Spain: Phenolic compounds by HPLC-MS/MS, cytotoxicity and biological potential on nitric oxide radicals and lipoxygenase inhibition. Journal of Functional Foods, 2024, 124, 106631
AGROVOC subjects
MalusManzana
Polifenoles
Flavonoides
Antinflamatorios
Lipoxigenasa
Other field subjects
AntinflamatoriosCromatografía de líquidos (uv, luminiscente, ms, electroquímica, etc)
Flavonoides
lipoxigenasa
MALUS
Manzana
Polifenoles
Sponsorship
APPLECUT (ref. PID2019-108081RR-C21) and APPLEDIV (PID2022-
141847OR-C31, PID2022-141847OR-C33) projects, financed by the
2019 and 2022 calls of the Ministry of Science and Innovation oriented
to challenges of society.