Influence of the FecX(R) Allele in Heterozygous Ewes on Follicular Population and Outcomes of IVP and ET using LOPU-Derived Oocytes
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Date
2013
Authors
Lahoz Crespo, BelénAlabart Alvarez, José Luis
Folch Pera, José
Sánchez Ruiz, Pilar
Echegoyen Pérez, Elías
Cocero Oviedo, Mª Jesús
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Abstract
Contents Ewes heterozygous for the FecX(R) allele (R+) in the bone morphogenetic protein 15 (BMP15) gene display increased ovulation rate and prolificacy. Besides this phenotypic advantage, the influence of the FecX(R) allele on follicle number and size, oocyte competence and in vitro production (IVP) remains undefined. With these aims, 8 R+ and 8 wild-type (++) ewes were subjected to 2 laparoscopic ovum pick-up (LOPU) trials (four sessions per trial; two with and two without FSH) and subsequent IVP and fresh embryo transfer. All follicles >3mm were punctured (n=1673). Genotype did not significantly affect the number of punctured follicles per ewe and session (10.4 and 10.2 in R+ and ++ untreated ewes, 17.4 and 14.3 in R+ and ++ FSH-treated ewes, respectively), but follicular diameter of R+ ewes was significantly reduced compared with ++ ewes (-0.2mm in untreated and -0.8mm in FSH-treated ewes; p<0.01). R+ ewes showed higher recovery rate and increased numbers of total and suitable cumulus-oocyte complexes for in vitro maturation (IVM). Similar rates of day 8 blastocysts were observed in R+ (36.1%, 147/407) and ++ (32.6%, 100/307) ewes, but the final output of day 8 blastocysts per ewe and session was higher in R+ ewes (+0.75; p<0.005), without differences in survival rate at birth of the transferred embryos (40.4%, 21/52 vs 36.4%, 16/44, respectively). In conclusion, a higher number of oocytes proven to be competent for in vitro development and embryo survival after transfer are recovered from R+ ewes, despite the lower mean size of their follicles at puncture.
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Lahoz, B.; Alabart, J. L.; Folch, J.; Sanchez, P.; Echegoyen, E.; Cocero, M. J. Influence of the FecX(R) Allele in Heterozygous Ewes on Follicular Population and Outcomes of IVP and ET using LOPU-Derived Oocytes. Reproduction In Domestic Animals, 2013, 48, 5, 717-723
AGROVOC subjects
OvinoHeterocigoto
Ovocito
In vitro
Embrión animal
Reproducción animal
Other field subjects
AllelesAnimals
BMP15
Bone morphogenetic protein 15
BOOROOLA
cloprostenol
Embryo Transfer
Female
Fertilization in Vitro
Flurogestone Acetate
Follicle Stimulating Hormone
Gene
Heterozygote
Hormones
INCREASED OVULATION RATE
Luteolytic Agents
MORPHOGENETIC PROTEIN 15
Mutations
oocyte retrieval
Oocytes
OVUM PICK-UP
progestins
SECRETED FACTORS
Sheep
Sterility
Sponsorship
This work was supported by Mº Ciencia e Innovacion (TRACE PET-
2008-0076 project) and INIA (grant for B. Lahoz).