Influence of the FecX(R) Allele in Heterozygous Ewes on Follicular Population and Outcomes of IVP and ET using LOPU-Derived Oocytes

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dc.bibliographicCitation.endpage723es_ES
dc.bibliographicCitation.issue5es_ES
dc.bibliographicCitation.stpage717es_ES
dc.bibliographicCitation.titleReproduction In Domestic Animalsen
dc.bibliographicCitation.volume48es_ES
dc.contributor.authorLahoz Crespo, Belénes_ES
dc.contributor.authorAlabart Alvarez, José Luises_ES
dc.contributor.authorFolch Pera, Josées_ES
dc.contributor.authorSánchez Ruiz, Pilares_ES
dc.contributor.authorEchegoyen Pérez, Elíases_ES
dc.contributor.authorCocero Oviedo, Mª Jesúses_ES
dc.coverage.spatialCiencia animales_ES
dc.date.accessioned2025-01-31T12:36:29Z
dc.date.available2025-01-31T12:36:29Z
dc.date.issued2013es_ES
dc.date.updated2025-01-31T12:09:29Z
dc.description.abstractContents Ewes heterozygous for the FecX(R) allele (R+) in the bone morphogenetic protein 15 (BMP15) gene display increased ovulation rate and prolificacy. Besides this phenotypic advantage, the influence of the FecX(R) allele on follicle number and size, oocyte competence and in vitro production (IVP) remains undefined. With these aims, 8 R+ and 8 wild-type (++) ewes were subjected to 2 laparoscopic ovum pick-up (LOPU) trials (four sessions per trial; two with and two without FSH) and subsequent IVP and fresh embryo transfer. All follicles >3mm were punctured (n=1673). Genotype did not significantly affect the number of punctured follicles per ewe and session (10.4 and 10.2 in R+ and ++ untreated ewes, 17.4 and 14.3 in R+ and ++ FSH-treated ewes, respectively), but follicular diameter of R+ ewes was significantly reduced compared with ++ ewes (-0.2mm in untreated and -0.8mm in FSH-treated ewes; p<0.01). R+ ewes showed higher recovery rate and increased numbers of total and suitable cumulus-oocyte complexes for in vitro maturation (IVM). Similar rates of day 8 blastocysts were observed in R+ (36.1%, 147/407) and ++ (32.6%, 100/307) ewes, but the final output of day 8 blastocysts per ewe and session was higher in R+ ewes (+0.75; p<0.005), without differences in survival rate at birth of the transferred embryos (40.4%, 21/52 vs 36.4%, 16/44, respectively). In conclusion, a higher number of oocytes proven to be competent for in vitro development and embryo survival after transfer are recovered from R+ ewes, despite the lower mean size of their follicles at puncture.en
dc.description.sponsorshipThis work was supported by Mº Ciencia e Innovacion (TRACE PET- 2008-0076 project) and INIA (grant for B. Lahoz).
dc.description.statusUnpublishedes_ES
dc.identifier.citationLahoz, B.; Alabart, J. L.; Folch, J.; Sanchez, P.; Echegoyen, E.; Cocero, M. J. Influence of the FecX(R) Allele in Heterozygous Ewes on Follicular Population and Outcomes of IVP and ET using LOPU-Derived Oocytes. Reproduction In Domestic Animals, 2013, 48, 5, 717-723
dc.identifier.issn09366768
dc.identifier.urihttp://hdl.handle.net/10532/7466
dc.language.isoenes_ES
dc.relation.doihttps://doi.org/10.1111/rda.12150es_ES
dc.relation.urihttps://doi.org/10.1111/rda.12150es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Spaines_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Españaes_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/es_ES
dc.subject.agrovocOvinoes_ES
dc.subject.agrovocHeterocigotoes_ES
dc.subject.agrovocOvocitoes_ES
dc.subject.agrovocIn vitroes_ES
dc.subject.agrovocEmbrión animales_ES
dc.subject.agrovocReproducción animales_ES
dc.subject.otherAlleles
dc.subject.otherAnimals
dc.subject.otherBMP15
dc.subject.otherBone morphogenetic protein 15
dc.subject.otherBOOROOLA
dc.subject.othercloprostenol
dc.subject.otherEmbryo Transfer
dc.subject.otherFemale
dc.subject.otherFertilization in Vitro
dc.subject.otherFlurogestone Acetate
dc.subject.otherFollicle Stimulating Hormone
dc.subject.otherGene
dc.subject.otherHeterozygote
dc.subject.otherHormones
dc.subject.otherINCREASED OVULATION RATE
dc.subject.otherLuteolytic Agents
dc.subject.otherMORPHOGENETIC PROTEIN 15
dc.subject.otherMutations
dc.subject.otheroocyte retrieval
dc.subject.otherOocytes
dc.subject.otherOVUM PICK-UP
dc.subject.otherprogestins
dc.subject.otherSECRETED FACTORS
dc.subject.otherSheep
dc.subject.otherSterility
dc.titleInfluence of the FecX(R) Allele in Heterozygous Ewes on Follicular Population and Outcomes of IVP and ET using LOPU-Derived Oocytesen
dc.typeJournal Contribution*
dc.type.refereedRefereedes_ES
dc.type.specifiedArticlees_ES

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