Simultaneous determination of carotenoids, tocopherols, retinol and cholesterol in ovine lyophilised samples of milk, meat, and liver and in unprocessed/raw samples of fat.
Loading...
Files
Date
2018-03-10
Authors
Bertolín Pardos, Juan RamónJoy Torrens, Margalida
Rufino Moya, Pablo José
Lobón Ascaso, Sandra
Blanco Alibés, Mireia
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier B.V.
Typology
texto , revista , artículo , artículo originalAbstract
An accurate, fast, economic and simple method to determine carotenoids, tocopherols, retinol and cholesterol in lyophilised samples of ovine milk, muscle and liver and raw samples of fat, which are difficult to lyophilise, is sought. Those analytes have been studied in animal tissues to trace forage feeding and unhealthy contents. The sample treatment consisted of mild overnight saponification, liquid-liquid extraction, evaporation with vacuum evaporator and redissolution. The quantification of the different analytes was performed by the use of ultra-high performance liquid chromatography with diode-array detector for carotenoids, retinol and cholesterol and fluorescence detector for tocopherols. The retention times of the analytes were short and the resolution between analytes was very high. The limits of detection and quantification were very low. This method is suitable for all the matrices and analytes and could be adapted to other animal species with minor changes.Copyright © 2018 Elsevier Ltd. All rights reserved.
Description
Keywords
Bibliographic citation
Bertolín, J. R., Joy, M., Rufino-Moya, P. J., Lobón, S., & Blanco, M. (2018). Simultaneous determination of carotenoids, tocopherols, retinol and cholesterol in ovine lyophilised samples of milk, meat, and liver and in unprocessed/raw samples of fat. Food Chemistry, 257, 182-188. https://doi.org/10.1016/j.foodchem.2018.02.139
AGROVOC subjects
Leche de ovejaColesterol
Retinol
Tocoferol
Carotenoide
Sponsorship
Agradecimiento al personal del CITA de Aragón. Este estudio ha sido financiado con fondos del FSE, del Gobierno español (INIA RZP2013-00001-00; contratos de M. Blanco, J.R. Bertolín y P.J. Rufino) y del Gobierno de Aragón (A49; beca-contrato de S. Lobón).